Senior Research Scientist - Target Validation, Early Oncology

Cambridgeshire, England
11 Feb 2021
11 Mar 2021
R & D , Scientific
Full Time
Contract Type
Experience Level
Experienced (non-manager)

At AstraZeneca, we turn ideas into life changing medicines. We're passionate about the potential of science to address the unmet needs of cancer patients; we work together to discover, develop, manufacture and market medicines that make a positive impact on the lives of patients as well as their families and communities.

These new and exciting positions will be working in the newly built Target Validation team in Oncology Bioscience. The team is responsible for the biological validation of targets coming from internal and external CRISPR screens or disease biology hypothesis to translate them into new drug targets for our oncology pipeline.

We are seeking two Research Scientists with a background in molecular and cellular biology, expertise in CRISPR and cell assays. A broad technical knowledge is required to assay the function of genes involved in different signalling and biosynthesis pathways.

As a Senior Scientist you will be a key member of a team passionate about target validation; you will bring your theoretical and practical expertise in signalling pathways and oncogenic drivers to establish in vitro assays and deliver datasets with direct impact on target decisions.

In this lab-based position you will develop and run in vitro models and cell-based assays to understand target biology. You will generate compelling pre-clinical experimental data to validate novel targets exploiting cancer vulnerabilities and synthetic lethality. This role will provide you with the opportunity to contribute to the discovery of new drug candidates with a wide variety of projects spanning different area of cancer biology.

Key Responsibilities:

  • Working in this laboratory-based position, you will design, develop, conduct and analyse in vitro experiments to assess the function of candidate targets and dissect their mechanism of action. A great foundation in cell biology and depth of expertise in techniques for target validation is crucial for this role.
  • Generation and analysis of genetically defined in vitro models (gene KO, overexpression and mutation; NGS and molecular biology methods)
  • Designing, performing and interpreting cell biology and mechanism-of-action studies such as proliferation assays, protein assays (western blot, FACS and immunofluorescence assays); mRNA assays (RT-PCR, qPCR, RNASeq)
  • Developing, troubleshooting and implementing cell-based assays
  • As a highly organised, self-motivated individual with a strong experimental background, you will stay up to date with relevant technical and scientific expertise in tumour biology and have a genuine passion for this field of research
  • Generating and maintaining clear experimental records
  • Presentation of experimental results to project teams
  • Focused and able to work to timelines

Essential skills:

  • Ph.D in Molecular or Cell biology or related field, with significant proven experience
  • Experience of cell culture and patient-derived models
  • Experience in molecular biology, to include cloning, sequencing, PCR; CRISPR and genome editing.
  • Experience in a range of cell biology methods/techniques e.g. transfection and transduction, generation of engineered cell lines, flow cytometry, proteomic assays, transcriptomics assays, assessment of cellular signalling.
  • Hands-on experience with a variety of in vitro assays to deconvolute gene functions
  • Understanding of critical steps in target validation
  • Passion for experimental work and capable of scouting for scientific and technical developments in the field of target validation
  • Positive and collaborative standout colleague with ability to learn new skills/ technologies
  • Experience preparing written scientific reports and oral presentations

Desirable skills:

  • Practical experience of delivering target validation projects
  • Good publication records in peer-reviewed journals
  • Background in Cancer Biology
  • Excellent communication and networking skills with experience of interacting optimally across subject areas and expertise.

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